Distinctions Between International Law And Municipal Law

Distinctions Between International Law And Municipal Law George, the Chief Justice of Ruretania, has been invited to sit on the ICJ as an ad hoc judge in a case now before the Court. After many years dealing with domestic law issues, he is now experiencing some difficulty in assessing the material submitted to the Court by the parties. These materials include: a. An article in the American Journal of International Law written by an eminent jurist; b. Draft articles prepared by the International Law Commission; c. A decision of the Supreme Court of one of the parties to the dispute; d. A United Nations General Assembly Resolution; e. A Treaty to which only one of the parties to the dispute is a party; f. A statement by the foreign minister of one of the parties; and g. A previous decision of the ICJ. Draw up a detailed memorandum for George explaining the sources of modern international law and advising him in particular as to the weight he should attach to the above-mentioned materials as sources and evidence of international law, drawing upon doctrinal aspects, relevant court judgments and opinions of authors. Introduction One of the primary points of distinction between international law and municipal law may be drawn from the nature of their sources. International law has been described by authors as a decentralized system which arguably lacks a predefined and concrete structure which discriminates between potential sources as to their importance and value in the resolution of issues  [1]  . The international judge does not have reference to a constitution, codes of law or statutes as in the municipal scenario. This is due to the fact that in international law there is no compulsory jurisdiction and any rules which a state must abide to are the same rules which it has expressly consented to, either by way of its consistent practice in the formation of custom or else expressly in the formation of treaties. Technically speaking, such contractual arrangement usually creating mutual obligations by way of treaty are the only source of international which is by its very nature objectively binding upon states, a reflection of the pacta sunt servanda principle in domestic Civil Law. It is for this reason that the law of treaties has been held to be a primary principle for centuries. Custom, on the other hand is primarily a matter of evidence. Brownlie  [2]  speaks of a categorization between the formal and the material sources of law. The formal sources are the legal procedures and methods for the creation of rules of general application which are legally binding on the addressee such as international custom created by constant state practice and the law contained in treaties. The material sources, on the other hand, provide evidence of the existence of rules which, when proved, have the status of legally binding rules of general application, here one can include decisions of international courts or tribunals, resolutions of the General Assembly of the United Nations and possibly Doctrinal sources. Brownlie continues to argue that in reality, save the usefulness in academia, th e so-called elevation of formal sources do not really exist in international law  [3]  as this would create a hierarchy which is not actually present. The Statute of the ICJ  [4]  constitutes a generally accepted list of the sources of international law ascribed to by the court  [5]  : 38 (1)The Court, whose function is to decide in accordance with international law such disputes as are submitted to it, shall apply: a. international conventions, whether general or particular, establishing rules expressly recognized by the contesting states; b. international custom, as evidence of a general practice accepted as law; c. the general principles of law recognized by civilized nations; d. subject to the provisions of Article 59, judicial decisions and the teachings of the most highly qualified publicists of the various nations, as subsidiary means for the determination of rules of law. An article in the American Journal of International Law written by an eminent jurist In order to help the Chief Justice of Ruretania in the performance of the new role of ad hoc judge in a case before the International Court of Justice, we need to give guidance on how to assess and use the contents of an article published in the American Journal of International Law written by an eminent jurist, that was submitted by the parties as part of the materials to be used as part of the deliberations of the judges. Article 38 of the Statute of the International Court of Justice lists the traditional sources of International Law, which include the international conventions of a general or a particular nature that are recognised by the parties to the case, international custom that is evidenced by general use and acceptance at law, general principles of law as accepted by civilised nations and as subsidiary means to establish these rules of law, the judicial decisions and the teachings of the most highly qualified jurists of the various nations. At the time of drafting, the term convention was used to mean any sort of agreement, however with the passage of time this term was redefined to make a specific reference to multilateral treaties. Without any doubt these written, accepted and published agreements are the primary source of law-making at the international law level. The only situation where any other rule takes precedence over a treaty is where the rules forms part of the jus cogens doctrine. Jus cogens is a normative rule that is generally accepted by the majority of states and from which no other state can expect to be given a derogation. It is also argued that the international custom as evidence of a general practice that is accepted by law is of equal validity and importance to the contents of treaties as new customs may replace previously agreed treaties and new treaties replace older customs. Article 38(d) of the ICJ statute specifically states that judicial decisions and the teachings of the most highly qualified publicists of the various nations may be used as subsidiary sources and the court is entitled to refer to these publications. This is subject to the provisions of Article 59 of the same statute. Article 59 states that the decision of the International Court of Justice shall have no other bearing or binding force except on the parties of the case in question. However as part of the courts decision, reference to other judicial decisions and to previously published works of eminent writers may be made. The works of prominent jurists and scholars are accepted only as secondary or subsidiary sources of international law but can be considered to be an essential element in the development of the rules that are included treaties, custom and the general principles of the law. More recently, the acts or resolutions of the various international organizations are also being considered as secondary sources of international law. Draft articles prepared by the International Law Commission In 1946, the UN General Assembly passed a resolution to set up a committee of legal experts to pass on recommendations to the UN Secretary General on the ways the General Assembly could proceed in the development of codification of international law. This Commission recommended that the UN sets up a permanent Commission with the same task. Thus in 1947 the UN set up the International Law Commission which has as its objectives the codification of international law as well as solving international legal problems  [6]  . The International Law Commission prepares drafts, according to Section 20 of its Statute  [7]  , which are requested by the General Assembly or by government, by an inter-governmental organization or by a UN agency or on its own initiative. When the matter is brought to its attention by the General Assembly, one of its members sits with the Commission as a Special Rapporteur. In order to finalise a draft the Commission prepares a plan of work, receives written opinions and suggestions from the governments and then puts these together into a final draft. This final draft is submitted to the General Assembly for its final approval. B.G. Ramcharan  [8]  describes the International Law Commission as the vehicle for systematizing consolidating, up-dating, adapting, and expanding the law, and for giving an opportunity to the States [] to participate in this process. In The Work of the International Law Commission  [9]  any draft is given a dual aspect. On the one hand there is the codification of existing law as well as the formulation of what the Commission considered to be desirable developments in the field. How effective are draft articles for George? Although draft articles prepared by the International law Commission are a good indication of the general opinion on an issue in the international sphere (due to the opinions submitted by the States), these provisions are not binding. Therefore a provision in a Convention or a decision by the ICJ has more binding effect. In fact the 1952 draft convention on arbitral procedure, although it was described as unexceptionable and as perfect from a technical point of view, it still was not accepted as the basis of a convention as the members of the General Assembly saw it as over-ambitious  [10]  . Conventions concluded by the UN on the basis of drafts prepared by the ILC include the Vienna Convention on the Law of Treaties, Vienna Convention on the Law of Treaties between States and International Organizations or between International Organizations, Vienna Convention on Succession of States in respect of State Property, Archives and Debts and others. Drawing a comparison between the draft articles of one of the above and the actual Convention, one can easily see that most of the draft articles are adopted into the Convention word by word. I have carried out this exercise for the first twenty articles of the Vienna Convention on the Law of Treaties. In this comparison I have found that the substance of the articles has to a large extent been carried on into the Convention. Moreover the General Assembly introduced four other articles but removed only one whole article and two sub-articles. From this exercise I can conclude that the draft articles, before the Convention was enforced, were a very good indication of the opinio juris on the matter especially due to the fact that states give their opinion prior to the drawing up of a draft. Therefore my advice to George would be that when he has to decide on a specific matter, first he should look into written legal provisions especially Conventions which have the most binding effect. If his findings are lacking he ought to see if the ILC has drawn up draft articles on the matter (since these are a codification of the unwritten customary law). Otherwise George has to look at the other sources of international law such as court decisions and the opinion of publicists on the matter as laid down in Article 38 of the Statute of the International Court of Justice. A decision of the Supreme Court of one of the parties to the dispute As the name indicates, the Supreme Court is the most important organ of the judiciary of the nation. However, within the international sphere, this position is taken up by the International Court of Justice. This does not reduce the importance of the Supreme Court, but its authority is merely superseded by a more senior court, which regulates conduct between states. Nevertheless, there is a theoretical issue which divides this are into two schools of thought: dualism and monism. Dualist doctrine points to essential differences between international law and municipal law. This consists primarily in the fact that local legislation differs from international law in a fundamental way as international law regulates law between sovereign states whilst municipal law applies solely within a state and regulates the relationship between the citizens of a state and its executive.  [11]  This theory was espoused particularly by the German jurist Heinrich Triepel, who argued that in case of conflict between municipal and international law, a court would apply municipal law.  [12]   On the other hand, monism was espoused by jurists who believed in the supremacy of international law even within the municipal sphere. International law is seen as the best available moderator of human affairs, and a logical condition of the legal existence of states.  [13]   However, these theories were criticized on the basis that they hold conflicting views with the way that international courts ought to behave. Nevertheless, a state cannot refrain from its international obligations citing municipal law, as provided in Article 27 of the Vienna Convention which states that A party may not invoke the provisions of its internal law as justification for its failure to perform a treaty.  [14]   Municipal law is also relevant in international law as has been demonstrated in numerous cases. In fact, many instances in international law require concordance with municipal law to function correctly, such as in cases related to extradition and protection of human rights, as well as treaties. Municipal law is important to such an extent that it can be used to determine cases in international tribunals. In the Serbian Loans Case  [15]  , the Permanent Court considered whether it had jurisdiction under its statute in a case where the point at issue was a question which had to be decided by the application of municipal law. It concluded that the duty of the Court was to exercise jurisdiction when two states agree to have recourse to the Court. The Court weighted the case and decided to apply Serbian law in this case. The role of municipal law in international law was closely examined in the case concerning Certain German Interests in Polish Upper Silesia.  [16]  In this case, the Court observed that it was not called upon to interpret Polish law, but there was nothing to prevent the Court from judging whether Poland was acting in conformity with the Geneva Convention. Brownlie states that this statement indicates that municipal law can be considered as something which can create international responsibility. The general proposition is that international tribunals can take account of municipal laws only as facts to further discussion.  [17]   Brownlie also states that in the practice of the International Court and other international tribunals the concept of municipal law as mere facts had six distinct aspects  [18]  . These aspects are: Municipal law may be evidence of a violation of a rule of treaty or a customary law Judicial notice does not apply to matters of municipal law, as the international tribunal will require proof of the existence of municipal law In accordance with the Serbian Loans case, interpretation of municipal law by national courts is binding on an international tribunal Municipal law must be applicable to an extent within international tribunals International tribunals cannot declare the invalidity of municipal law and respect domestic jurisdiction International law may designate a system of domestic law as the applicable law  [19]   Thus, given that in some cases international courts may apply municipal law, George must not discard totally the decisions of the Supreme Court, but must use it in order to help him with the case he is assigned to. Nevertheless, international law still supersedes municipal law, as provided in Article 27 of the Vienna Convention. The United Nations General Assembly Resolution Hon. Chief Justice, in order to understand better what is a United Nations General Assembly resolution, it would be ideal to start by providing you with a short and background information on the General Assembly itself. The United Nations General Assembly is one of the five principal organs  [20]  of the United Nations and the only one in which all member nations have equal representation. This means, that since there are 192 member states in the United Nations, each and one of them is a member of the General Assembly. Each member of the General Assembly has one vote and important matters such as the elections of members, peace and security, budgets, and the suspension/discharging of members, is done by a two-thirds majority of those present and voting. Other recommendations which are considered to contain less weight, are decided by a majority voting. As stated in the official website of the UN, the General Assembly meets under its president or secretary general in regular yearly sessions, the main part of which lasts from September to December and resumed part from January until all issues are addressed (which often is just before the next sessions start). It can also meet for special and emergency issues. The Presidency rotates annually and the President is voted for by the same members of the United Nations. It is customary that the largest and most powerful countries, such as China, do not hold the presidency. On the other hand, countries such as Luxemburg and Switzerland, although small, had the presidency in the 1970s. It is therefore important to point out that even Malta held the Presidency back in the 1990, when the general assembly elected Dr. Guido De Marco as president of its forty-fifth session. Although the power of the President is limited, he may accomplish much through his personal influence and political adeptness.  [21]   The UN General Assembly Resolution The General Assembly votes on many resolutions brought forward by member states. It is interesting to appreciate that since 1945, at least 9229 resolutions spanning a vast and varied range of international issues have been adopted by the General Assembly.  [22]   Whilst this record of the General assemblys formal concern, considerations and expression of views is indeed impressive, the fact remains that, except for budget and membership questions, General Assembly resolutions are not legally binding on member states.  [23]   The legal competence of the General Assembly to consider legal matters flows from the UN Charter. Article 10 gives the General Assembly the authority to discuss and make recommendations on any matter within the scope of the Charter, either to the United Nations membership generally or to the Security Council in particular.  [24]   In addition, the charter imposes two major restrictions on the General Assemblys powers to discuss and make recommendations. The first is embodied in the principle set out in Article 2, paragraph 7 of the charter, which prohibits the United Nations to intervene in matters which are essentially within the domestic jurisdiction of any state and the second restriction is found in Article 12 of the Charter, which states that the General Assembly shall not make any recommendation while the Security Council is exercising its functions in respect to any international dispute. However, although this might suggest that the General Assembly has no powers at all, resolutions have frequently been the origin of multilateral treaties drafted and promulgated under UN auspices.  [25]  General Assembly declarations had sometimes evolved into conventions adopted by the international community. Prominent among these declarations are those dealing with the use of forces back in the 1960s.  [26]   Hon. Chief Justice, it is therefore important for you to know that back in the 1970s Malta was also involved in a very important resolution, embedding principles related to the seabed and its resources. In fact, it was Arvid Pardo, a Maltese diplomat, who initiated a fifteen year process which led to a near-unanimous passage of General Assembly Resolution 2749  [27]  . The principles brought forward in the resolution were later incorporated in the Law of the Sea Convention. This eventually proves that although resolutions are not binding, they may influence the nature and substance of international law in many ways and may also have legal force if they are regarded as statements of customary international law. The General Assembly thus is not a World legislature. It does not codify laws or norms even though they are adopted unanimously and without any formal opposition. General Assembly resolutions are merely recommendations however the General Assembly can function as an instrument to collect and shape up into tangible form the international communitys customary norms. State practice then becomes the main factor determining whether General Assembly resolutions give rise to new norms of international law or remain merely recommendations. Such new norms subsequently are codified into recognized principles of International Law through the promulgation of special conventions adopted by the General Assembly and approved by the required number of parties. A Treaty to Which only one of the parties to the dispute is a party A treaty is an international agreement concluded between states in written form and governed by international law, whether embodied in a single instrument or in two or more related instruments and whatever its particular designation  [28]  . They are generally held to be an ever-increasingly important source of international law and are a key towards the ends of developing a system of peaceful cooperation amongst states irrespective of their domestic legal particularities  [29]  . Since 1945, over 33,000 treaties have been deposited with the UN  [30]  . In Art.38(1)(a), the Statute of the International Court of Justice makes reference to international conventions establishing rules which are expressly recognised by the contesting states which may either be general or particular in their application. This article gives a very wide definition which seems to include not only bilateral and multilateral treaties but also any form of international agreement in any form. It often happens that the language of a treaty which is presented before the court as a document containing rules recognised by the states in dispute is not always manifestly clear and precise as to make it clear whether such treaty is actually applicable to the circumstances of the case. The court will proceed to interpret the instrument, therefore determining its scope and effect and applicability to the dispute in question. According to Art.36(2)(a) of the Statute of the ICJ, states may at any time and by common accord, recognise as compulsory the jurisdiction of the court in the resolution of several legal disputes which might arise between them, amongst these, the Statute mentions the interpretation of a treaty. In any case, the Vienna convention lays down that treaties must always be interpreted in good faith and in accordance with the ordinary meaning of the words contained within, given their context within the treaty  [31]  . The norm in public international law is that a treaty creates rights and obligations only between its parties, therefore third party states are not bound by any treaty which they have not individually entered into. This notion is best expressed by the maxim pacta tertiis nec nocent nec prosunt  [32]  . Art.34 of the Vienna Convention states that a treaty does not create either obligations or rights for a third state without its consent. This reasoning had already been confirmed in the earlier case dealing with the German Interests in Polish Upper Silesia  [33]  back in 1925. In this case, the PCIJ stated that a treaty only creates law as between states which are parties to it; in case of doubt, no rights can be deduced from it in favour of third states. There are however some slight exceptions to this otherwise general principle. A norm which was originally confined to a treaty between a limited number of states may eventually become part of international custom through state practice and therefore achieve general application  [34]  . The position at international laws appears to be clear enough in regard to cases where obligations are imposed on states which are merely third parties two treaties entered into by other states. The situation may tend to differ in cases which concern the granting of rights to third parties. The question is over when, if at all, such a right becomes complete and enforceable by the third party state. According to Brownlie and McNair amongst others, the general rule is that the third state only benefits in this sense if it expressly or implicitly assent to the creation of the right.  [35]  The corollary is that the third state may refuse the right either explicitly or else simply by failing to exercise the alleged right. On the other hand, if a third state has accepted a right, the right cannot be revoked by the actual parties to the treaty if it can be established that the right conferred was not originally intended to be revocable, save the third states freedom to consent to such revocation or limitation  [36]  . Strictly speaking, in the case brought before Judge George the matter of there being a treaty to which only litigant (A) is a party does not have a bearing on the relationship between states A and B in litigation since B is clearly a third party to the treaty and is therefore outside the scope of any rights or obligations created or conferred by the treaty provided that B had not consented to any obligations created by the treaty or else accepted any rights arising from the same. A statement by the foreign minister of one of the parties Parliamentary statements are one of the modes to detect a state practice and the position a state takes in the international plane. The foreign minister on a number of occasions may be asked to elucidate the states position on a particular issue of an international concern, thus parliamentary statements, can evoke a particular state practice. A state practice is a general practice which is normally accepted as law. The other limb is that this state practice must be accepted as law and this is what we call opinio juris sive necessitatis. This opinio juris sive necessitatis is what authors refer to as the psychological element of states, this is because the state practice has to be accompanied by the intent which represent why the state acts in that particular manner. This means that parliamentary statements and therefore ministers statements in the international court of justice reflects and determine the state practice and what its position is. The Position of the Minister in the ICJ Another important thing that we must notice is that the International Court of Justice deems the position of foreign Ministers to be of a very important nature. This is illustrated in Belgium VS Congo delivered by the International Court of Justice on 14th Febuary 2002 where it implied that customary international law grants to foreign ministers as long as they hold their office the advantage of absolute jurisdiction and inviolability. The reason behind this is for the international court of justice to ascertain performance of the very importatnt functions that foreign ministers excercise when they represent their states. This clearly points out that the international court of justice gives a lot of importance to the position of the foreign minister in the international court of justice but the question that arises is Does it give the same importance to their statements? One must begin by saying that for a statement of foreign minister to be accepted and taken into consideration it must be in accordance with international law. In fact a point that was revealed in Military and Paramilitary Activi

Define Biotechnology and Write an Essay on the Current Status of Plant Biotechnology in the 21st Century by Focusing Mainly on Genetic Engineering

Define biotechnology and write an essay on the current status of plant biotechnology in the 21st century by focusing mainly on genetic engineering O O O FAQ | Register Now | Sign In HOME PHYSICAL SCIENCESPHYSICS SPACE CHEMISTRY APPLIED PHYSICS AEROSPACE OPTICS EARTH SCIENCESENVIRONMENT ENERGY ATMOSPHERIC PALEONTOLOGY GEOLOGY OCEANOGRAPHY LIFE SCIENCESGENETICS & MOLECULAR BIOLOGY EVOLUTION MICROBIOLOGY ECOLOGY & ZOOLOGY IMMUNOLOGY NEUROSCIENCE MEDICINECANCER RESEARCH PUBLIC HEALTH PHARMACOLOGY CLINICAL RESEARCH AGING VISION SOCIAL SCIENCESANTHROPOLOGY ARCHAEOLOGY PSYCHOLOGY SCIENCE EDUCATION & POLICYSCIENCE HISTORY PHILOSOPHY & ETHICS CULTURETECHNOLOGY MATHEMATICS SCIENCE & SOCIETY SPORTS SCIENCE RANDOM THOUGHTS HUMOR VIDEO CONTRIBUTORS Home > Life Sciences > Genetics & Molecular Biology > Humboldt Fellow and Science Recent advances in plant biotechnology: Applications in Agriculture. By Ashwani Kumar | August 21st 2009 11:29 AM | Print | E-mail | Track Comments Humboldt Fellow and Sc ience More Articles †¢Climate change and green economy-a cleaner option for sustainability †¢Sitopaladi churna is an ayurvedic medicine for cough and cold †¢Ethnobotany and Ocimum sanctum (Tulsi)All Articles About Ashwani Professor Emeritus ,Former Head of the Department of Botany, and Director Life Sciences, University of Rajasthan, Jaipur. 302004, India At present†¦ View Ashwani ‘s Profile Ashwani Kumar Recent advances in plant biotechnology: Applications in Agriculture. Ashwani Kumar Professor of Botany, Department of Botany and P G School of Biotechnology University of Rajasthan Jaipur 302004. [email  protected] com Tel 0141 2711654 ( Off) 0141 2654100 ( Res) Mob (0) 9414057484 Abstract:Biotechnology is an area of production and research in which biological systems and biological principles are employed to solve technological problems. In this sense it becomes all inclusive. And during the last decade the advancements in biology have led to the develo pment newer areas like , cellular engineering, biochips and biomaterial science, stem cells, nanobiotechnology etc. Biotechnology is a vast subject and covers Gene and genome analysis: analysis of genes and gene networks showing the potential for industrial application; gene expression studies; biotech plant breeding, e. g. marker assisted breeding.Transgenic technologies: Production and analysis of transgenic crops; gene insertion studies; gene silencing; factors affecting gene expression; post-translational analysis; molecular farming; field trial analysis; commercialisation of modified crops; safety and regulatory affairs Functional genomics: bioinformatics; gene function studies for applied uses Comparative genomics: applications to crop species; use of current crop databases Physiological studies: pathways relevant to an application; secondary metabolites; manipulations of physiology for stress resistance – abiotic and biotic stress resistance including salinity and drou ght stress.Development of salt resistance plant using plant biotechnology. Host pathogen interaction and role of plant biotechnology for developing resistant corps Developmental studies: developmental mechanisms leading to a further understanding of an industrial use of plants. Plant tissue culture and its role in plant biotechnology. Introduction: Scientists have been improving plants by changing their genetic makeup since the late 1800s. Typically, this has been accomplished through crossbreeding and hybridization, in which two related plants are cross-fertilized and the resulting offspring have characteristics of both parent plants.In the breeding process, however, many undesirable traits often can appear in addition to the desirable ones. Some of those undesirable traits can be eliminated through additional breeding, which is time consuming. Breeders can then further select and reproduce the offspring that have the desired traits. Many of the foods that are already common in our diet are obtained from plant varieties that were developed using conventional genetic techniques of breeding and selection. Today, by inserting one or more genes into a plant, scientists are able to produce a plant with new, advantageous characteristics.The new gene splicing techniques are being used to achieve many of the same goals and improvements that plant breeders historically have sought through conventional methods. They give scientists the ability to isolate genes and introduce new traits into foods without simultaneously introducing undesirable traits. This is an important improvement over traditional breeding. Because of the increased precision offered by the bioengineered methods, the risk of introducing detrimental traits is actually likely to be reduced. Gene and genome analysis:Detailed studies have been conducted on plant genome and physical and genetic maps are available for several plants. As an example of studies on genome the rice genome is discussed here. Rice has a much smaller genome (430 Mbp per haploid genome) than many other crops that belong to the Poaceae family. Due to the genome colinearity, high similarity in gene order and gene content, among the Poaceae family, the importance of rice genetics has been emphasized, and comparative analyses among rice, wheat, and maize have been intensively studied.As a result, rice becomes the model crop for the molecular genetic approach. This crop is available for many applications, including the construction of a high dense map, expressed sequence tag (EST) and full genomic sequence database, bacterial and yeast artificial chromosome (BAC and YAC) libraries, quantitative traits loci (QTL) mapping for yield and morphology, functional genomics by knockout mutagenesis using T-DNA insertion, map-based cloning, and genetically modified rice using transformation techniques. ( see review Cho et al. 2007 cited in Kumar and Sopory 2007).State-of-the-art Genome Profiling (GP) : The traditional approach for species identification is exclusively based on phenotypic traits such as morphological, anatomical, chemical properties and others, which are often affected by environmental factors and thus are difficult to analyze and unreliable. Interspecies homogeneity, intraspecies variability and the existence of undescribed species often lead to phenotypic misidentification. Moreover, species, which are phenotypically far less prominent, cannot be always identified in this way.To overcome these problems, genotypebased (nucleic acid-based) techniques have been employed as an alternative or complementary approach and have continuously been developed including RFLP ,AFLP, RAPD, 16S rRNA or 16S-23S internal transcribed spacer (ITS) sequence analysis and others. These methods provide a possible way to identify species directly based on their genomic sequences but none of them have been shown to identify species in general, mainly because of the insufficiency in the amount of information which they can provide.In this stream, the whole genome sequencing is surely the most definitive solution for species identification though simply too redundant for such purposes and impossible in practice to analyze all the constituents of a heavily dense population. On the other hand, the information obtained from the comparison of a single gene is often not sufficient to place a species at the appropriate position on the phylogenetic tree.In order to deal with above issues, previously Nishigaki and co-workers have described a realistic solution conforming to the notion of the amount of information sufficient for species identification and demonstrated this by inventing a novel method called Genome Profiling (GP), which is a temperature-gradient gel electrophoresis (TGGE) analysis of random-PCR products. Next, the complexity of the generated data, genome profiles, can be simplified by extracting feature points in GP, i. e. species identification dots (spiddos) which can be used for fur ther processing of measuring the similarity of two species by calculating Pattern Similarity Score (PaSS). Further, the technical advances by constructing internet-based GP databases (named On-web GP), and developing a highly reproducible and miniaturized system (micro-TGGE) have moved this technology towards being a universal, general and global tool for species identification( see review Biyani 2007 cited in Kumar and Sopory 2007)..Recombinant DNA technology: Gene targeting (GT) Gene targeting (GT) is a key technology for the rational, accurate and safe exploitation of plantsthrough genetic manipulation. Moreover, it offers the potential to completely knockout the expression of target genes or to make specific changes to gene function, objectives that cannot be achieved by conventional transgenesis. The ability to target DNA integration would permit the locus-specific integration of a transgene into a predetermined ite of the host genome, avoiding the accidental inactivation of an endogenous gene localized at the insertion site or the unexpected expression profiles of the transgene itself, the so-called position effect. Systematic isolation and sequencing of genomic DNA flanking the insertion sites (known as FSTs or Flanking Sequence Tag offers the opportunity to rapidly characterize plants altered in a candidate gene sequence. This approach is notably most useful in fully sequenced genomes such as in Arabidopsis thaliana.With 125 Mbp and 26,422 genes, the Arabidopsis genome shows very limited synteny with the 420–466 Mbp and 60,000 predicted genes of the rice genome. The recombination machinery has been well conserved throughout evolution, as an essential component of cell survival. In nature, homologous recombination is a DNA maintenance pathway that protects chromosomes against damage affecting both DNA strands, such as double strand breaks (DSBs) or interstrand crosslinks. DSB repair (DSBR) has been one of the most investigated homologous repair p athways see SHRIVASTAVA1, SHARMA2 AND KUMAR 2007 cited in Kumar and Sopory 2007).Recently plastid genome transformation technique has gained prominence due to its better integration and less chances of random spread. The genome propagated by higher plant plastids, the plastome, is typically a double stranded DNA molecule of 130 to 160 kb. Over one hundred copies of this genome can be present in a single plastid. It is ideally represented as a circular monomer containing 2 inverted repeats, even though reality is more complex since linear and circular multimers have been frequently detected The complete sequence of this highly polyploidy genome is available for about 20 different species of angiosperms http://megasun. ch. umontreal. ca/ ogmp/projects/other/cp_list. html). The first successful transformation of tobacco was performed using as marker a mutant plastid DNA fragment covering the 16SrRNA gene derived from a line resistant to spectinomycin and streptomycin. Major improvement s in the selection process were soon obtained with the dominant aadA marker gene, inactivating spectinomycin or streptomycin. When fused to GFP, this marker can be used to track the selection process.Genes encoding resistance to kanamycin, nptII and more recently aphA-6 are also possible options, and could be more appropriate for some species (Kumar et al. , 2004a see review Dubald. 2007 cited in Kumar and Sopory 2007). There are three different fates for the external DNA to get integrated into the native genome. They are homologous recombination, illegitimate recombination or nonhomologous end joining, and single-strand annealing. . Single-strand annealing (SSA), a third path of repair, requires the presence of repeated sequences on both sides of a break.After exonuclease degradation of the 5’ ends, repair occurs by annealing of the two complementary sequences, a process leading to the loss of the genetic information contained between these repeats. With respect to the speci es preferential DSB repair pathway, HR but also IR mediates transgene integration. This second aspect explains the inefficiency of GT in higher plants, which use HR as a minor pathway of repair. Thus, despite the fact that transgene integration processes are still unclear in plants transgenic DNA would be preferentially integrated by end joining whether or not sharing homology within the host genome.GM crops: The U. S. Food and Drug Administration (FDA) has found no evidence to indicate that either ordinary plant deoxyribonucleic acid (DNA) or the DNA inserted into plants using bioengineering presents food safety problems. Nor are the small amounts of the newly expressed proteins likely to change dramatically the safety profile of the plant. If safety concerns should arise, however, they would most likely fall into one of three broad categories: allergens, toxins, or anti-nutrients. FDA has extensive experience in evaluating the safety of such substances in food.It is important to n ote that the kinds of food safety testing typically conducted by developers of a bioengineered food crop to ensure that their foods meet all applicable requirements of the Food, Drug and Cosmetics Act (FD;C Act) address these potential concerns. In the event that something unexpected does occur, this testing provides a way to detect such changes at the developmental stage and defer marketing until any concern is resolved. As aforementioned, some of the food safety concerns that could arise include: Allergens: Foods normally contain many thousands of different proteins.While the majority of proteins do not cause allergic reactions, virtually all known human allergens are proteins. Since genetic engineering can introduce a new protein into a food plant, it is possible that this technique could introduce a previously unknown allergen into the food supply or could introduce a known allergen into a â€Å"new† food. Toxins: It is possible that a new protein, as introduced into a cr op as a result of the genetic modification, could cause toxicity. Anti-nutrients: It is possible that the introduction of anti-nutrients, such as molecules like phytic acid, could reduce essential dietary minerals such as phosphorus.The use of genetic engineering techniques could also result in unintended alterations in the amounts of substances normally found in a food, such as a reduction of Vitamin C or an increase in the concentration of a naturally occurring toxicant in the plant food. LEGAL AND REGULATORY ISSUES: One important component in ensuring food safety is the U. S. regulatory structure. The FDA regulates bioengineered plant food in conjunction with the United States Department of Agriculture (USDA) and the Environmental Protection Agency (EPA).FDA has authority under the FD;C Act to ensure the safety of all domestic and imported foods for man or animals in the United States market. The exceptions to this are meat, poultry and certain egg products, which are regulated b y USDA. The safety of animal drug residues in meat and poultry, however, is regulated by FDA. Pesticides, including those bioengineered into a food crop, are regulated primarily by EPA. USDA’s Animal and Plant Health Inspection Service (APHIS) oversees the agricultural and environmental safety of planting and field testing bioengineered plants.Bioengineered foods and food ingredients must adhere to the same standards of safety under the FD;C Act that apply to their conventionally bred counterparts. This means that these products must be as safe as the traditional foods in the market. FDA has the power to remove a food from the market, or sanction those marketing the food if the food poses a risk to public health. It is important to note that the FD;C Act places a legal duty on developers to ensure that the foods they market to consumers are safe and comply with all legal requirements.Area under the commercialization of genetically modified (GM), often called biotech crops con tinued to grow for the ninth consecutive year at a sustained double-digit growth rate of 20% in 2004 (James, 2004). The estimated global area of approved GM crops for 2005 was 90. 0 million hectares with $4. 70 billion global market value—based on the sale price of GM seed plus any technology fees that apply. The global value of the GM crop market is projected a more than $5. 0 billion for 2005 (James, 2004). Commercialization of genetically modified (GM) crops continued to grow for the ninth consecutive year.It reflects the substantial improvements in productivity, the environment, economics, health and social benefits realized by farmers, consumers and society. At the same time the growing controversy over GM food products increased interest in food labelling and identity preservation (IP) of GM crops. Hence, an IP system must be designed to provide assurances that the desired traits are present (or absent) in a product from the seed source, through all steps of production and delivery, to the end user. There are numerous regulatory issues related to GM crops.These include the testing and acceptance of new GM crops for commercial introduction, both domestically and internationally. Nearly every country has different approaches and many have their own regulatory framework, with an intent to prevent cross-contamination of the conventional food and feed industries. IP tracking software is also available in market to ease the burdens associated with precise record-keeping requirements. The economics of IP has been calculated by various scientists depending on different applied IP systems.Niche-marketing opportunities will grow, because of the availability of GM crops and finally, IP of agricultural commodities from GM crops can provide greater choice and value desired by both agricultural producers and consumers (DOSHI AND FRANCOIS EUDES, 2007 cited in Kumar and Sopory 2007) Based on annual percentage growth in area, of the eight leading GM crop countries , India had the highest percentage year-on-year growth in 2004 with an increase of 400% in Bt cotton area over 2003, followed by Uruguay (200%), Australia (100%), Brazil (66%), China (32%), south Africa (25%), Canada (23%), Argentina (17%) and USA at 11%.India increased its area of approved Bt cotton, introduced only two years ago, from approximately 38,038 hectares in 2002/ 03 to 560,000 hectares in 2004/05 seasons with Bt coverage of 11. 65% and approximately 300,000 small farmers benefited from Bt cotton( see review DOSHI AND EUDES, 2007 cited in Kumar and Sopory 2007). . REGULATORY ISSUES RELATED TO GM CROPS: There are numerous regulatory issues related to GM crops. These include the testing and acceptance of new GM crops for commercial introduction and the introduction of food products containing ingredients from GM crops, both domestically and internationally.Nearly every country has different approaches and many have their own regulatory framework. Regulation is a very dynami c issue with changes being reviewed and proposed in many countries on an ongoing basis. Numerous regulatory actions are consequently being proposed as governments react to consumer concerns and pressures. Several countries have or have proposed to create new agencies to specifically cover GM crops. Approaches range from cautious acceptance to attempts to ban (growing and even imports), at least for the foreseeable future, all crops and products with GM traits.Each is approaching the testing, introduction, and acceptance of GM crops in its own manner and on its own time schedule. Table 6 summarizes the current status of some of the regulations related to introduction, approval, and commercial acceptance of GM crops. Table 6. Status of regulations over GM products. Abiotic and biotic resistance: Coat Protein Mediated Resistance: CP is an important structural protein as it not only protects the viral nucleic acid from degradation, but also plays an important role in virus infection.Its functions includes acquisition and transmission of virus by vectors, cell to cell and long distance spread of the virus in host plants, and for some viruses, it regulates one or more steps of virus replication. Coat Protein (CP) mediated resistance has been demonstrated for 17 groups of viruses, and so far this strategy has shown best promise. CP transgenes have been shown to be effective in preventing or reducing infection and diseases caused by homologous and closely related viruses (Gonsalves, et al. , 1998).Coat protein-mediated protection has been reported for Tobacco mosaic virus, TMV,Tomato mosaic virus, ToMV, (Sa), Cucumber mosaic virus, CMV, Alfalfa mosaic virus, AlMV, (Loesch-Fries et al. , 1987; Tumer et al. , 1997), Potato virus X, PVX, Potato virus Y, PVY, Potato leaf roll virus, PLRV, Papaya ringspot virus (PRSV) and a number of other viruses. CP-mediated resistance in Cantaloupe, Papaya, Potato, Squash and Tomato has been tested under the field conditions with fair d egree of protection against most of the viruses (Table 4). ( see review Verma and Parveen 2007 cited in Kumar and Sopory 2007)Anti-HIV Agents Among Desert Plants Around 40 million people are affected due to the Human Immuno-deficiency Virus globally. During the past decades, a large number of anti-viral screening experiments on medicinal plant extracts have been reported and have led to the selection of several extracts active towards herpes viruses. A promising result of a naturally occurring antiherpetic agent was given by n- docosanol (a natural 22 carbon saturated fatty alcohol) which is undergoing phase III clinical trials in patients. Clinical testing of the topical formulation, or systemic administration of drug suspensions has demonstrated good therapeutic index, since high doses of n- docosanol do not elicit appreciable toxicity. The findings show that natural products are still potential sources in the search for new antiherpatic agents (Hattori et al. , 1995). Various pla nt extracts used in Ayurvedic medicine for inhibitory effects on HIV virus have been studied (Hattori, personal communication). A large number of such plants occur in semi-arid and arid climate of Rajasthan. Acquired immunodeficiency syndrome (AIDS) , the great pandemic of the second half of the 20th Century, is still a threatening disease world wide.Many research approaches are currently aimed at developing novel agents to arrest the replication of HIV through various targets. These may include the inhibition of reverse transcriptase (RT), protease (PR), membrane fusion and integrase. HIV PR enzyme has been demonstrated to play an essential role in viral replication ( see review Kumar 2007 cited in Kumar and Sopory 2007) A range of HIV PR inhibitors have been designed and applied in clinical trials such as Sanqunavir, Ritonavir and Indinavir. However, the development of drug resistance by virus, irrespective of the target, remains as an overwhelming problem in AIDS chemotherapy.Thu s there is great need to search for and develop new and different anti-HIV candidates from plants and natural products are of considerable importance. In search for anti-HIV active agents from natural products, many attempts at screening traditional medicines have been made. Biotic and abiotic stress: Environmental abiotic stress conditions, and especially drought and salinity, are currently the major factors which reduce crop yields world-wide leading to the fact that more than 800 million people are chronically undernourished..The United Nations Environment Program estimates that approximately 20% of agricultural land and 50% of cropland in the world is saltstressed This salinity, in particular, is an increasing problem and nearly half of the area under irrigation, is at risk to be lost due o building up of salinity. Therefore genetic improvement of salt tolerance has become an urgent need for the future of agriculture in arid and semiarid regions.. One way of solving this problem would be breeding tolerant varieties of crop plants that can be grown on saline soils, but these breeding programs are time consuming and remained elusive .Hence, many metabolic changes are known to occur in plants subjected to salt stress, physiological parameters such as ionic relations have been suggested to be used as tolerance indicators since they can be related to salt tolerance mechanisms. Biofuel production: India has over 180 million of wasteland out of which 90 million ha is uncultivable. The degraded and denuded lands arise due to soil erosions as well as secondary salinizations. However Calotropis procera is a potential lant for bioenergy and biofuel production in semi arid regions of the country because it is able to grow on such lands. The plant has a growth potential of 2 dry tones to 40 dry tones per ha depending on the agro climatic conditions of it’s growth. The plant has high level of regeneration potential and could be harvested up to 4 times a year. The plant yields valuable hydrocarbons which could be converted into diesel substitutes. The bio-diesel derived from Calotropis procera is free from NOx gases, S02 and Suspended Particulate Matter (SPM) and has high cetane value.Due to it’s enormous potential for growth under adverse climatic conditions Calotropis procera is suggested as potential plant for bio-diesel production under semi-arid and arid conditions ( Anita and Kumar,2005, Anita et al. 2005). Jatropha curcas also provides non-edible oil which could be converted into methyl ester with a gain of glycerine ( Anita and Kumar 2007) . The JME is used as mix in the ratio of 05, to 20 percent blend to fossil fuel diesel and in Germany the use of Rape seed methyl ester is increasing. Soil salinity: Soil salinity affects plant production in many parts of the world, particularly on irrigated land.NaCl is the predominant salt in most saline environments. Many crop species are sensitive to high concentrations of salt with nega tive impacts on agricultural production. Maize (Zea mays L. ) is considered a moderately salt-sensitive plant.. Salt resistance of plants is a complex phenomenon that involves biochemical and physiological processes as well as morphological and developmental changes.. In addition to general osmotic stress, high concentrations of Na+ are toxic to maize and molecular mechanisms for salt resistance have not been fully identified or characterized (Zoerb et al 2004).The analysis of the plant’s proteome is an important amendment to the analysis of the genome, because gene expression is altered under salinity stress. The proteome, in contrast to the genome, is not static but rather dependent on a number of responses influenced by internal and external factors. The plant adaptation to environmental stress, such as soil salinity, is expected to have a strong influence on proteins. One approach to study the molecular mechanisms of plant responses to salinity is to use 2D polyacrylamide gel electrophoresis. Furthermore, the dentification of differentially regulated proteins can lead to the identification of proteins and their corresponding genes which are involved in the physiology of salt resistance. The high resolution achieved by 2D gels and computer-assisted analysis of the differentially regulated proteins were used to examine those proteins whose synthesis was modulated by salt treatment and to quantify these changes. As far as we know, our investigations are the first to characterize the differentially expressed proteins from roots and shoots of maize after treatment with low and high salt stress.Plant material was an efficiently Na+-excluding maize inbred line developed in our laboratory. According to Munns the growth response to salt stress consists of two phases, first, a water-deficit that results from the relatively high solute concentrations in the root medium and, second, ion-specific stress resulting from altered K+/Na+ ratios or Na+ and Cl? concent rations that are toxic to plants. The aim of this study was to elucidate biochemical and physiological reactions of glycophytes to salt stress in the first phase of salinity. While all major crops, as well as most wild species, are glycophytes, i. e. ensitive to relatively low salt concentrations, there are plants naturally adapted to conditions of high salinity in the soil. These plants, known as halophytes, include a large taxonomic variety and occupy diverse habitats, from extreme dry to temporarily waterlogged sites or salt marshes, and can tolerate NaCl concentrations similar, or even higher than that of sea water, ca. 500 mM (Figure 1). It is – without doubt – necessary to develop sustainable biological production systems which can tolerate higher water salinity because freshwater resources will not come up with increasing demands of agricultural practice in near future.The sustainable use of halophytic plants is a promising approach to valorize strongly salinise d zones unsuitable for conventional agriculture and mediocre waters. The development of cash crop halophytes and the breeding of salt resistant crop varieties will require a clear understanding of the complex mechanisms of salt stress tolerance, which we are still lacking despite intensive research during the last decade (see review KOYROAND HUCHZERMEYER 2007 cited in Kumar and Sopory 2007).Recombinant proteins: It has been estimated that 1 g of recombinant antibody could be produced in leaves of a plant crop for only about US$100 while the current prices for monoclonal antibodies range from US$2000 to US$5000 per gram. Indeed the cost of producing 1 kg of recombinant protein from most major field crops is estimated to be 10 to 50 times lower than the cost of producing the same amount by E. coli fermentation. Whole plants also have an advantage when tissues such as a fruit, tuber, etc. an be used to express the protein of interest (James and Lee 2001), and an area of undisputed adva ntage occurs when the oral delivery of pharmaceuticals, as well as feed and food enzymes, is possible. However, there are also some evident obstacles that arise when a whole plant is used for large-scale protein production( see review SODERQUIST and LEE 2007). Cell-free systems have proved to have high utility at the genomic, transcriptomic and proteomic levels and to form a vital component of many aspects of recombinant gene expression, and of both structural and functional proteomics..Compared with DNA microarrays, protein bio-chips provide more challenges and have yet to be perfected due to the complexity and inherent difficulties with protein immobilization. Novel cell free translation system is unique discovery: A novel cell-free translation system is described in which template-mRNA molecules were captured onto solid surfaces to simultaneously synthesize and immobilize proteins in a more native-state form. This technology comprises a novel solid-phase approach to cell-free tra nslation and RNA–protein fusion techniques.A newly constructed biotinylated linker-DNA which enables puromycin-assisted RNA–protein fusion is ligated to the 3? ends of the mRNA molecules to attach the mRNA-template on a streptavidin-coated surface and further to enable the subsequent reactions of translation and RNA–protein fusion on surface. The protein products are therefore directly immobilized onto solid surfaces and furthermore were discovered to adopt a more native state with proper protein folding and superior biological activity compared with conventional liquid-phase approaches.We further validate this approach via the production of immobilized green fluorescent protein (GFP) on microbeads and by the production and assay of aldehyde reductase (ALR) enzyme with 4-fold or more activity. The approach developed in this study may enable to embrace the concept of the transformation of ‘RNA chip-to-protein chip’ using a solid-phase cell-free trans lation system and thus to the development of high-throughput microarray platform in the field of functional genomics and in vitro evolution (Biyani et al. 2006). Plant tissue culture: Another area of biotechnology is micropropagation of plants.The aim of this technique is a fast production of a great number of genetically identical plants from a highly valuable mother plant or e. g. monosexual male and female plants. These plants can be either directly sold on the market for planting, used for breeding purposes, for genetechnology or the technique is used as a method for basic science studies. Using petiole explants from transgenic plants containing the auxin responsive MAS promoter linked to the GUS reporter gene (Fig. 15, 16) the distribution of auxin within the cultured petiole could be followed during the induction phase of somatic embryogenesis (Neumann 2000 and Neumann 2006).Interestingly, the cells forming the glandular canal contain high concentrations of auxins as shown by using transgenic plants containing the auxin sensitive MAS-promoter coupled to the GUS-gene ( Fig see below ), whatever the significance. Rhizogenic centers develop near vascular bundles prior to those embryogenic centers. Fig. 2 Plasmid pPCV812 with the MAS promoter and the GUS reporter gene, hyg=Hygromycin resistance, Ap/Cb=Ampicillin/Carbenicillin resistance (courtesy of Dr. Z. Koncz, Max-Planck- Institut Cologne, Germany, for providing the plasmid) Genetic factors play a central role to induce somatic embryos, i. . to provide the competence of the species for the process. Here, great variation can be found even within a genera such as Daucus. Eight of twelve Daucus species cultured in identical conditions produced somatic embryos (D. halophilus, D. capillifolius, D. commutatus, D. azoricus, D. gadacei, D. maritimus, D. maximus, D. carota ), whereas four species (D. montevidensis, D. pussillus, D. muricatus, D. glochidiatus) were not competent to do so. Under identical culture co nditions, only 8 out of 12 species and subspecies of the genus Daucus proved capable of somatic embryogenesis.Random amplified polymorphic DNA analysis indicated a polymorphism between the genomes of individual species that were capable of embryogenesis and those that were not. Two specific bands (1. 1 kbp, 0. 68 kbp) were detected only inthe genomes of individuals with the capacity for embryogenesis. These were cloned and sequenced, and the homology of the nucleotide sequences of the various species was detected: this ranged from 74% to 92% for the larger sequence and from 92% to 97% for the smaller one. These DNA sequences would appear to be useful as a marker of the capacity for somatic embryogenesis in the genus Daucus (Imani et al. 001) The sequences obtained in this study have been registered with the European Bioinformatics Institute (EMBL). The access numbers for the sequences are: AJ278039 DCA78039; AJ278040 DCA78040; AJ278041 CA78041; AJ278042 DCA78042; AJ278176 DCA278176; AJ278177 DCA278177; AJ278178 DCA278178; AJ278179 DCA278179. No open reading frames were detected. We performed later additional studies with other Daucus species (D. capillifolius; D. carota ssp. Azoricus and gadecaei) as shown in Table 1 to determine the use-fulness of these RAPD products as markers for identifying the ability of Daucus species to generate somatic embryos (Fig. b). There was a 100% correlation between the embryogenic potential of the species (Table 1) and the occurrence of the 1. 1-kbp and 0. 68-kbp band (Imani et al. 2001). Micropropagation Technique in Enhancing the Productivity of Crops have been taken up at large scale at TERI ( see review Saxena, 2007 see Kumar and Shekhawat 2007) Some of the activities undertaken at MTP include: †¢ Large-scale production of superior quality planting material of various economically important plant species using tissue culture technology †¢ Mass multiplication of those species which are difficult to regenerate by co nventional methods f propagation or where conventional methods of propagation are inadequate to meet the demand of planting material †¢ Development of new micropropagation protocols and refining of others so as to make them suitable for large-scale production of plants †¢ Helping the entrepreneurs/industry through technology transfer, mother cultures and training of staff †¢ Assisting clients in setting-up their own tissue culture labs †¢ Creating awareness Till date over 15 million plants of forest species, cash crops, medicinal and aromatic plants, and ornamentals (foliage and flowering) have been dispatched to various state forest and horticulture epartments, private entrepreneurs, nurseries, farmers etc. for field demonstrations and routine plantations. In addition, MTP is in possession of micropropagation protocols for over 90 economically important plant species. Field demonstration plots of tissue cultured plants have been laid at different locations to ev aluate and compare their growth performances with conventional plants. Besides transfer of technologies to industry for commercialization, MTP has been instrumental in capacity building and creating awareness about tissue culture technology through seminars/ workshops/training programmes, exhibitions, etc. Dhawan and Saxena 2004; Saxena and Dhawan, 2004). Secondary metabolites: Since the establishment of plant tissue culture techniques in 1960’s, significant contributions have been made to the development of biochemical studies on secondary metabolism such as structural elucidation, biosynthesis, enzymology, metabolic regulation system, intracellular distribution of metabolites and relevant enzymes, metabolite transportation, molecular biology and many others . However, one of the greatest difficulties and challenges in the application of plant tissue culture to metabolism research has been that unorganized callus tissues have often failed to ccumulate metabolites usually det ected in the mother plant. In some cases, metabolic potential was recovered through the development of a production medium, change in culture conditions or selecting cell strains of high productivity (Fujita and Tabata, 1987). It is commonly observed that recalcitrant callus tissues begin to synthesize secondary metabolites after organ – such as shoots and roots – differentiation . Although somatic embryogenesis occurs in cultured cells of numerous plant species, it has rarely been applied to secondary metabolite production. Recently secondary metabolite production by somatic embryo ultures and especially by those of Corydalis species has been reviewed by HIRAOKA and Bhatt, 2007) . References: Anita Kumari and Ashwani Kumar (2005) SOME POTENTIAL BIOFUEL PLANTS FOR SEMI-ARID AND ARID REGIONS AND IMPROVING THEIR GROWTH AND PRODUCTIVITY In : CARRASCO J. E. , L. SJUNNESSON, P. HELM, A. GRASSI (eds) BIOMASS FOR ENERGY, INDUSTRY AND CLIMATE PROTECTION. pp 279-281. Anita Kuma ri, Ashwani Kumar, V. R. Kumar (2005) PRODUCTIVTY OF CALOTROPIS PROCERA IN SEMI-ARID REGIONS OF RAJASTHAN AND ITS USE AS RENEWABLE SOURCE OF ENERGY In : CARRASCO J. E. , L. SJUNNESSON, P. HELM, A.GRASSI (eds) BIOMASS FOR ENERGY, INDUSTRY AND CLIMATE PROTECTION. pp 276-278 Dhawan V and Saxena S (2005) Production of superior quality disease-free planting material. In: Chadha KL, Ahloowalia Imani, J. , (1999): In situ- Nachweis der Auxinverteilung in kultivierten Petiolenexplantaten von transgenen Pflanzen wahrend der Induktion der somatischen Embryogenese bei Daucus carota L. Diss. Justus Liebig Universitat, Gie? en, Germany J. Imani †¢ L. Tran Thi †¢ G. Langen,B. Arnholdt-Schmitt †¢ S. Roy †¢ C. Lein †¢ A. Kumar K. -H. Neumann (2001) Somatic embryogenesis and DNA organization of genomes from selected Daucus species.Plant Cell Rep 20:537–541 Prasad, BS KV and Singh SK (Eds. ) Crop Improvement and Production Technology of Horticultural Crops Proceedings of First Indian Horticulture Congress – 2004. pp 174-184. Kumar A. (2004) Calotropis Procera: a Potential Plant for Hydrocarbons from Semi-Arid and Arid Regions In : Van Swaaij, Fjallstrom, Helm and Grassi (eds):. Biomass for energy, industry, and climate protection. Proceedings of the Second World Conference ETA-Florence, Rome Italy WIP-Munich , Germany pp 173. Kumar, A. and Sudhir Sopory ( eds) ( 2007) Recent advances in plant biotechnology.IK International New Delhi Kumar A and N S Shekhawat ( eds) (2007) Plant tissue culture, Molecular markers and their role in agriculture production. IK International. New Delhi Neumann, K. -H. (1995): Pflanzliche Zell- und Gewebekulturen. Ulmer Verlag, Stuttgart,304 pages Neumann KH (2000) Some studies on somatic embryogenesis, a tool in plant biotechnology. http://bibd. uni-giessen. de/ghtm/ 2000/uni/p000004. htm Neumann, K. -H. (2006): Some studies on somatic embryogenesis: a tool in plant biotechnology. In: Kumar and Roy (eds) Plant b iotechnology and its applications in tissue culture.I. K. International, New Delhi . pp 1-14. Shekhawat V. P. S. and A. Kumar 2006 Somaclonal variants for salt tolerance and in vitro propagation of peanut. In: (Eds. ) A. Kumar, S. Roy and S. K. Sopory. Plant Biotechnology;its Application in Tissue Culture. I. K. International New Delhi, Mumbai, Bangalore. pp. 177-196 Shekhawat, V. P. S. , Kumar, A. , and K. H. Neumann. 2005. Bio-reclamation of secondary salinized soils using halophytes. Biosaline Agriculture ; Salinity tolerance in Plants. (Eds. ) M. Ozturk, Y. Waisal, M. A. Khan and G. Gork, Birkhauser Verlag , Switzerland. p 145-152. Shekhawat, V. P. S. , Kumar, A. , and K. H. Neumann. 2006. Effect of NaCl salinity on growth and ion accumulation in some chenopodiaceous halophytes. Communication in Soil Science and Plant analysis 13-14 (37), 1933 – 1946 Manish Biyani, Yuzuru Husimi, and Naoto Nemoto (2006) Solid-phase translation and RNA–protein fusion: a novel approa ch for folding quality control and direct immobilization of proteins using anchored mRNA Nucleic Acids Res. 2006 November; 34:140-. Saxena, S and Dhawan V (2004) Changing Scenarios in Indian Horticulture In : PS Srivastava, A Narula and SSrivastava (Eds. ) Plant Biotechnology and Molecular Markers. Anamaya Publishers, New Delhi. pp. 261-277. Related Articles on Science 2. 0 Applications of biotechnology in plant tissue culture Biotechnology and genomic tools Biofuels include Medicinal plant cultivation. Recent advances in plant biotechnology: Applications in Agriculture. Know Science And Want To Write? Register Now To Get Your Own Column! What's Happening Top Articles New Comments Events 1. 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The Biggest Myth About Persuasive Essay Exposed

The Biggest Myth About Persuasive Essay Exposed The History of Persuasive Essay Refuted When you revise your essay, you've got to make sure its organization is completely appropriate to your intended audience, the paper context, and the goal. Academic writers or essay writing providers ensure it is simple for students to find the things they need fast. Our qualified academic experts will aid you in finding a great persuasive essay topic according to all of your requirements and compose a custom-tailored model answer. When being graded on the standard of work presented, the instructors will often base the last grade around certain criterion. One of the most intriguing facets of a technology like Wikipedia is that it's built from tiny fragments of time that the technology allows to be composed into somethingits many flaws acknowledgedwhich is totally free and fundamentally helpful. In order to do so, you first need to create a persuasive essay outline and stick with it in order to have a crystal clear structure and strong supportive elements. No matter the subject, the structure is precisely the same for any persuasive essay. Aside from the fundamental format that can be learned from a template, one has to also understand the aim of the writing activity. To choose which subject you're likely to discuss, it's essential to see the complete collection of good persuasive speech topics from the special area of study. Quite simply, obtaining a focused topic is a must, but obtaining a specific audience is equally as important. There are lots of places where you can discover a valid outline sample but the very best reference you are able to consult is your professor. Though people believe education is a correct and will make society, overall, a better place for everybody, others feel there's no real method to provide a free college education as colleges would still have to be funded (likely through tax dollars). Then you're interested in figuring out how to compose persuasive paper. There are several persuasive essay topics to pick from to finish your high school or college assignment. Instead of high school subjects, college subjects are somewhat more troublesome to discover. Trying to persuade your teacher may be quite exhausting. The Appeal of Persuasive Essay Persuasive writing can be hard, especially whenever you're made to face with a close-minded audience. Cigarettes need to be more expensive. Before you are able to move on to writing your essay, it's crucial to structure your thoughts. Still, if you'd like your paper to hit the bulls-eye and modify the way that your reader thinks, you require a few tactics. You may make sure that you're a talented writer. Your audience should know precisely what the writer is going to debate and why. War is a horrible medium, which has been utilized for thousands and thousands of years, to reach certain goals throughout the use of force. If you would like to figure out how to compose a decent persuasive essay, you're looking in the proper place! You would then have an introduction that has the points you'll be making and a couple of words on just what the topic is all about. To begin with, it's important to pick a topic that it is possible to take a stand for. Generally speaking, you will be asked to opt for a perspective in an argument or maybe to support a particular viewpoint against critiques. To begin with, if you're arranging a persuasive speech, you ought to think about a topic that could create mental pictures in the minds of your audience. It's possible to locate a good deal of persuasive essays on the planet of politics. It's not sufficient to claim a single idea is far better than the other, you truly have to prove it. How to Choose Persuasive Essay Content Organization Content indexing and organizing in all sorts of essays is quite important. Containing all the appropriate information concerning the topic makes them handy and yet beneficial. Well, first, you are going to want to find good persuasive speech topics. Research, analysis and organization are just some of the elements that can enable you to compose an effective essay. Persuasive essays share a whole lot of resemblance with argumentative essays. Your persuasive essay will have a lot of paragraphs. Writing a superb persuasive essay is not an easy job, however, it's achievable. The very best persuasive short essays often concentrate on controversial problems.

Essay about Twists and Turns of the 1920s - 953 Words

Depression Era Project. Throughout the 1920’s there were many bad times and many good times. From things like Flappers, women who began to do things that were considered â€Å"Un-lady like†, to the Stock Market crash all the way to the election of Franklin D. Roosevelt, the 20’s and 30’s were full of twists and turns. Even the fact that Herbert Hoover, who was beloved during WWI, was beaten by newcomer Franklin D. Roosevelt was a surprise. Though, after the Stock Market had crashed FDR’s election and his many new policies was probably the best thing that had happened to America at the time, it seems other things, like Prohibition, Flappers and Speakeasies, tended to take the spotlight instead. There were many good things that occurred†¦show more content†¦They would set up secret places where people could secretly buy and drink alcohol without arousing suspicion. â€Å"To order alcohol without drawing attention or raising suspicion, barten ders asked customers to remain quiet and speak easy.† (Henderson para.7) These Speakeasies would even bribe the police with some of the alcohol being sold to keep them quiet. Apparently though the Prohibition was more of a problem about drunken workers, â€Å"A primary goal of 1920s Prohibition was the reduction of drinking by workers.† (Henderson para.4) So that raises the question as to why the sale of alcohol was banned entirely. But just as there were many wonderful times in the 1920’s there were quite a few bad times to go right along with them. Almost all of the bad things that happened in the 1920’s were brought by the Stock Market crash. 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